Identification of an up-accumulated polyamine oxidase 2 in pollen of self-incompatible ‘Wuzishatangju’ mandarin using comparative proteomic analysis
Yi Ren, Jiayan Pan, Zhike Zhang, Jietang Zhao, Xinhua He, Yonghua Qin and Guibing Hu
Scientia Horticulturae 266: 109279. (2020)
2020
บทคัดย่อ
Self-incompatibility (SI) is one of the main factors causing seedless fruits in Citrus. ‘Wuzishatangju’ (Citrus reticulata Blanco) is a natural bud sport from a self-compatible and seedy cultivar ‘Shatangju’. Our previous study showed that gametophytic SI caused seedlessness of ‘Wuzishatangju’. However, it is not clear what factors are responsible for its SI response. In this study, pollen tube growth kinetics was observed in the pistils of ‘Wuzishatangju’ using pollination combination of ‘Wuzishatangju’ (♀) × ‘Wuzishatangju’ (♂) and ‘Wuzishatangju’ (♀) × ‘Shatangju’ (♂). The protein profiles of pollen between ‘Wuzishatangju’ and ‘Shatangju’ were compared by quantitative proteomics analysis based on tandem mass tag technology. Pollen tube from self-pollination of ‘Wuzishatangju’ vigorously germinated in stigma while stopped elongating at the upper site of the style. Pollen from ‘Shatangju’ germinated and pollen tube reached the base of style, suggesting that the mutation plays a role in pollen of ‘Wuzishatangju’. Proteomic analysis indicated that a polyamine oxidase 2 (CrPAO2) was up-accumulated in pollen of ‘Wuzishatangju’ and catalyzed the spermine and spermidine oxidation leading to produce H2O2. The potential roles of CrPAO2 causing SI reaction in ‘Wuzishatangju’ were discussed.