Melatonin delays yellowing of broccoli during storage by regulating chlorophyll catabolism and maintaining chloroplast ultrastructure
Chenghao Wu, Shifeng Cao, Keqin Xie, Zongyu Chi, Jing Wang, Hongfei Wang, Yingying Wei, Xingfeng Shao, Chundan Zhang, Feng Xu and Haiyan Gao
Postharvest Biology and Technology, Volume 172, February 2021, 111378
2021
บทคัดย่อ
The effect of melatonin treatment on color, chlorophyll degradation and chloroplast structure in broccoli during storage was evaluated. One hundred µmmol L−1 melatonin markedly extended the shelf life of broccoli. Compared with control florets, melatonin treatment delayed the decline of chlorophyll content, and maintained more intact chloroplasts. Meanwhile, melatonin also suppressed the activities of chlorophyll catabolic enzymes such as chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PAO) and red chlorophyll catabolite reductase (RCCR), and down-regulated the expressions of BoNYC1, BoNOL, BoCLH, BoPPH, BoPAO, BoRCCR and BoSGR1 involved in chlorophyll catabolism. Taken together, our results indicated that melatonin could be a good candidate for delaying yellowing in broccoli during storage at 20 °C.