Identification of key genes involved in the two lignifications in stored king oyster mushrooms (Pleurotus eryngii) and their expression features
Yan Wang, Chaoyuan Xiang, Danqing Li, Minru Du, Yuli Mo, Zide Jiang and Jie Wang
Postharvest Biology and Technology, Volume 169, November 2020, 111289
2020
บทคัดย่อ
The enzyme genes involved in two lignifications of stored Pleurotus eryngii remain unclear. Here, we investigated the lignification profiles and associated gene expression by RNA-seq, molecular clone and qRT-PCR analysis to identify the key structural genes and reveal their expression features under different conditions. A total of 2803 differentially expressed genes (DEGs) were identified in samples at 1 °C compared to those at 4 °C, and 56 DEGs were annotated to lignification-related gene families. Ten lignification-related structural genes were cloned and only the expression patterns of PePAL1, Pe4CL1, Pe4CL3 and PePOD were proportionate to the change of lignification indexes of mushrooms during 4 °C storage. Their expression profiles at key time points during mushrooms growing, development, and two lignifications presented their consistency with that of lignin contents and implied relatively high expression in two lignifications. Expression profiles under cold and no-wounding treatments also showed correlation with two lignifications and presented their expression features. Namely, PePAL1 and PePOD could be stimulated by cold stress during the first lignification and triggered by wounding stress during the whole storage, meanwhile Pe4CL1 and Pe4CL3 could be induced by wounding stress during the first lignification and irritated by cold stress at the time point of the second lignification. These results showed that PePAL1, Pe4CL1, Pe4CL3 and PePOD were the key genes involved in two lignifications of stored P. eryngii and could be stimulated by wounding and cold stress at different time points of storage.