The Rlm1 transcription factor in Candida oleophila contributes to abiotic stress resistance and biocontrol efficacy against postharvest gray mold of kiwifruit
Yuan Sui, Zhiqiang Sun, Yuping Zou, Wenhua Li, Mingguo Jiang, Yuzhen Luo, Wenjian Liao, Yuanhong Wang, Xuewen Gao, Jia Liu and Huijun Wu
Postharvest Biology and Technology, Volume 166, August 2020, 111222
2020
บทคัดย่อ
Biological control utilizing antagonistic yeasts has been actively pursued as an alternative to synthetic fungicides for the management of postharvest diseases. Abiotic stress resistance is an important attribute for antagonistic yeasts, directly associated with their biocontrol efficacy. The MADS-box transcription factor, Rlm1, has been reported to regulate the response of model yeasts to cell wall stress. Rlm1 in the antagonistic yeast, Candida oleophila, was found to play a role in resistance to salt, heat, and oxidative stress. Two Rlm1 mutants (โRlm1-1 and โRlm1-2) were generated. Compared to the wild-type (WT), C. oleophila I-182, โRlm1-1, and โRlm1-2 were more sensitive to a variety of stresses, including heat, salt, and oxidative stress. The mutants also exhibited lower biocontrol efficacy against gray mold caused by Botrytis cinerea, and slower growth in kiwifruit wounds with respect to the WT. This study provided the information to understand the relationship between the Rlm1 transcription factor, stress resistance, and biocontrol efficacy of antagonistic yeasts used for the biocontrol of postharvest diseases.