Antifungal activity of the volatile organic compounds produced by Bacillus velezensis strains against postharvest fungal pathogens
Héctor CalvoI, sable Mendiara, Esther Arias, Ana Pilar Gracia, Domingo Blanco, María Eugenia Venturini
Postharvest Biology and Technology, Volume 166, August 2020, 111208
2020
บทคัดย่อ
It is known that Volatile Organic Compounds (VOCs), among several other mechanisms, are responsible for the antagonistic activity produced by microorganisms. In this work the volatilome of three biocontrol Bacillus velezensis strains (BUZ-14, I3 and I5) was tested in vitro and on fruit against Botrytis cinerea, Monilinia fructicola, M. laxa, Penicillium italicum, P. digitatum and P. expansum. In vitro, pathogens growth was significantly inhibited, in particular M. laxa, M. fructicola and P. italicum (66, 72 and 80 %, respectively) by BUZ-14 and B. cinerea (100 %) by I3 and I5, compared to the control. In vivo tests also showed significant inhibitions since volatile metabolites of I3 reduced grey mould in grapes by 50 % and those of BUZ-14 decreased brown rot severity in apricots, especially by M. fructicola, from 60 to 4โ€ฏmm. VOCs were identified by solid phase microextraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC–MS) and the antifungal activity of some of them was tested both in vitro and in vivo against the fungal pathogens. The main volatiles identified ranged from 12 to 15 compounds including 2-nonanone, 2-undecanone, 2-heptanone, 1-butanol, acetoin, benzaldehyde, butyl formate, diacetyl, nonane, or pyrazine, among others. Benzaldehyde and diacetyl obtained the lowest minimum inhibitory concentrations in vitro, ranging from 0.005 to 0.125โ€ฏmLโ€ฏL−1 depending on the pathogen tested. Moreover, diacetyl was able to control grey mould caused by B. cinerea in table grapes with only 0.02โ€ฏmLโ€ฏL−1 and to reduce blue rot in mandarins at the same dose up to 60 %. In this study, diacetyl and benzaldehyde have been identified as promising compounds to apply in active packaging during the postharvest commercialization of fruit. However, prior to the application, it is crucial to determine not only the active dose but also the phytotoxic, since some fruit species such as apples and apricots have proven to be highly sensitive.