Ethylene attenuates chilling injury of banana fruit via the MabHLH060/183 module in controlling phosphatidic acid formation genes
Ziyin Zhou, Yingying Yang, Wei Shan, Hui Zhang, Wei Wei, Jianfei Kuang, Jianye Chen and Wangjin Lu
Postharvest Biology and Technology, Volume 183, January 2022, 111724
2022
บทคัดย่อ
Chilling injury (CI) of banana represents a serious problem constraining postharvest preservation in the industry. Application of ethylene was found to efficiently attenuate CI of banana, however, the mechanism of which remains to be investigated. Here, we found that application of ethrel could ameliorate the CI of banana, as evidenced by lower CI index, electrolytic leakage and malondialdehyde content. Meanwhile, expression of phosphatidic acid (PA) formation genes MaPLDδ1, MaPLDδ5 and MaDGK2, as well as two transcriptional regulators MabHLH060 and MabHLH183 was significantly increased with the progression of CI, but decreased under ethrel treatment. MabHLH060 and MabHLH183 were shown to locate in nucleus and have transactivation ability. Importantly, these two transcriptional regulators MabHLH060/183 recognized the E-box elements in the promoters of MaPLDδ1, MaPLDδ5 and MaDGK2, and promoted their transcription. Together, these findings suggest that ethylene-alleviated CI of banana may involve the MabHLH060/183 module in modulating the expression of PA formation genes.