Inhibition effect of super atmospheric O2 packaging on H2O2-production and the key enzymes of ligin biosynthesis in fresh-cut Chinese cabbage
Ming Wen, Huihui Wang, Yulong Chen, Yueming Jiang, Feiping Chen and Zheng Luo
Postharvest Biology and Technology, Volume 159, January 2020, 111027
2020
บทคัดย่อ
Fresh-cut Chinese cabbage was packaged by super atmospheric O2, and held at 4โ€ฏ°C, taking air packaging as control. The contents of lignin and H2O2 and, the activities and transcription expressions of four key enzymes of lignin synthesis, i.e., phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA ligase (4-CL), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), were determined in the cabbage. The results showed that the lignin content of the cabbage packaged by super atmospheric O2 was markedly reduced (pโ€ฏ<โ€ฏ0.05) in comparison with control from day 3 to 12 during storage at 4 ºC. The H2O2 concentrations in fresh-cut Chinese cabbage under super atmospheric O2 was lower than that of control during storage at 4โ€ฏ°C and, significantly lower (p < 0.05) on day 8 of storage. The activities of PAL, 4-CL, CAD and POD under super atmospheric O2 packaging were significantly lower (pโ€ฏ<โ€ฏ0.05) than that of control from day 3 to 12 of storage at 4 ºC, except for 4-CL on day 9. Moreover, the transcript expressions of POD genes in the cabbage in super atmospheric O2 were dramatically reduced compared with control on day 8 of storage at 4โ€ฏ°C. It is suggested that the suppression of lignin synthesis of the cabbage under super atmospheric O2 packaging was attributed to the inhibition of H2O2, which feedback-regulated the key enzymes activities directly or indirectly.