Study on aflatoxin and sclerotium producing ability of Aspergillus flavus isolates
R. Heidarian, M. Javan-Nikkhah, A. Sharifi-Tehrani, K. Ghazanfari
Acta Horticulturae 963: 99-100. 2012.
2012
บทคัดย่อ
Some isolates of A. flavus produce aflatoxin on pistachio seeds. In this investigation 102 isolates of A. flavus were isolated from pistachio seed and processing residue samples collected from different areas of Kerman province. Aflatoxin producing ability was detected on Czapek dox agar medium with 0.3% â-cyclodextrin. The production of aflatoxin coincided with the presence of a bright blue fluorescent area surrounding colonies when observed under long wavelength (365 nm) UV light after 3 days of incubation at 26°C. Sclerotium producing ability was determined on Czapek dox agar, Czapek dox agar with 3% sodium nitrate and PDA medium. As a result 39.2% isolates produced aflatoxin and 15.7% isolates produced sclerotium. There was no relationship between aflatoxin and sclerotium producing ability and geographic distribution.