ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Ripening of tomato fruit locule gel tissue in response to ethylene

Mordy A. Atta-Aly, Jeffrey K. Brecht and Donald J. Huber

Postharvest Biology and Technology Vol: 19 Issue: 3 Pages: 239-244.

2000

บทคัดย่อ

Ethylene production by locule gel tissue excised from immature tomato fruit was inhibited by exposure to 4.5 mol l^-1 C₂H₄, and 1-aminocyclopropane-1-carboxylic acid (ACC) content was reduced. In contrast, CO₂ production, ethylene forming capacity (EFC), red colour development, tissue liquefaction and seed maturation as measured by subsequent germination rate were stimulated by C₂H₄, and, in immature gel, the onset of autocatalytic C₂H₄ production was hastened. The autoinhibition of C₂H₄ production in immature locule gel tissue required continuous exposure to C₂H₄, as transfer to air and then back to C₂H₄ resulted in C₂H₄ production first increasing to control levels, then decreasing again. Locule gel tissue from pink fruit however, responded to C₂H₄ treatment with increased C₂H₄ and CO₂ production, but ACC levels were unchanged. Inhibition of C₂H₄ action by pretreatment with STS inhibited both autoinhibition and autostimulation of C₂H₄ production in immature and mature gel tissues, respectively. These results indicate that there is a transition from a negative to a positive feedback mechanism of C₂H₄ on C₂H₄ biosynthesis in locule gel during ripening. Control of this feedback mechanism, which involves ACC synthesis, may be separate from that of other manifestations of C₂H₄ action.