Postharvest effects of acetaldehyde vapour on ripening-related enzyme activity in avocado fruit
Edna Pesis, Debora Faiman and Sasson Dori
Postharvest Biology and Technology Vol: 13 Issue: 3 Pages: 245-253.
1998
บทคัดย่อ
Exogenous application of acetaldehyde (AA) vapour to whole or half-peeled
avocado fruits (Persea americana cv. `Fuerte'), prior to storage,
inhibited fruit ripening. This inhibition was characterized by a delay in fruit
softening and a reduction in ethylene production. Activities of the cell wall
depolymerizing enzymes polygalacturonase (PG), -galactosidase
(
-GAL)
and endoglucanase (Cx), and of 1-aminocyclopropane-1-carboxylic acid oxidase
(ACC oxidase), were reduced immediately after treatment. AA-treated fruit had
reduced ethylene production and ACC oxidase activity, both in vivo and in
vitro. Levels of total free sulfhydryl (SH) group compounds increased in
AA-treated fruit, but diminished in untreated fruit. AA treatment inhibited
fruit pulp oxidation, while untreated control fruits oxidized and became brown.
Application of AA to a PG reaction mixture reduced PG activity on sodium
polypectate. Pre-incubation of the substrate with AA did not result in reduced
activity. Attempts to detect AA-bound proteins in AA-treated fruits were
unsuccessful.