Reduction in ethylene synthesis in parthenocarpic Actinidia deliciosa fruit induced by N-(2-chloro-4-pyridyl)-N'-phenylurea
Yoshinori Ikoma, Masamichi Yano, Zhong Chuan Xu and Kazunori Ogawa
Postharvest Biology and Technology Vol: 13 Issue: 2 Pages: 121-129.
1998
บทคัดย่อ
Parthenocarpic kiwifruit Actinidia deliciosa was produced and
induction of ethylene synthesis in response to ethylene treatment studied. At
anthesis, flowers were treated with N-(2-chloro-4-pyridyl)-N'-phenylurea
(CPPU). CPPU-treated parthenocarpic fruit and pollinated normal fruit were
harvested in early November, and then treated with 1000 l
l-1 ethylene at 20°C for 24 h. Ethylene
production following ethylene treatment was reduced and delayed in the
parthenocarpic fruit. To investigate the mechanism of this reduction in
ethylene synthesis, 1-aminocyclopropane-1-carboxylic acid (ACC) concentrations,
and gene expression of ACC synthase (using a cDNA probe, AD-ACS1) and of ACC
oxidase (with a cDNA probe, AD-ACO1) were analyzed. ACC concentrations and
levels in ACC synthase transcripts increased in the pollinated fruit 2 days
after the 24 h ethylene treatment, but not in the parthenocarpic fruit.
Accumulation of ACC oxidase transcripts was stimulated immediately by the 24 h
ethylene treatment in both pollinated and parthenocarpic fruit. These results
suggest that suppression of ethylene synthesis in parthenocarpic fruit resulted
mainly from suppressed expression of ACC synthase.