Beta-1,3-glucanase activity of two saprophytic yeasts and possible mode of action as biocontrol agents against postharvest diseases
Raffaello Castoria, Filippo De Curtis, Giuseppe Lima and Vincenzo De Cicco
Postharvest Biology and Technology. Volume 12, Issue 3, December 1997, Pages 293-300.
1997
บทคัดย่อ
The yeasts Rhodotorula glutinis (isolate LS-11) and Cryptococcus
laurentii (isolate LS-28), showing different levels of antagonistic
activity against a range of postharvest pathogens, were examined for their
possible mode(s) of action, in order to highlight the reasons for the higher
activity of isolate LS-28. Competition for nutrients appeared to play a role in
the activity of both yeasts, especially in the case of isolate LS-11. Direct
interaction with pathogen hyphae was shown only by cells of this same isolate,
whereas no interaction with fungal hyphae was observed for the more active
antagonist LS-28. The latter isolate was able to produce in vitro significantly
higher levels of extracellular -1,3-glucanase
activity than LS-11 when grown in the presence of hyphal cell walls of the
pathogens Penicillium expansum and Botrytis cinerea as sole
carbon sources. In our experimental conditions, antibiosis did not appear to be
involved in the activity of either antagonists.