ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

In vitro translation products of mRNA from pericarp tissue of tomato cultivars differing in chilling tolerance

Han-Ling Yu, Claude Willemot, Paul Nadeau, Serge Yelle and Yves Castonguay

Postharvest Biology and Technology Vol: 7 Issue: 3 Pages: 231-241.

1996

บทคัดย่อ

In vitro translation products from two tomato cultivars, Lycopersicon esculentum cv. `Early Cherry' (`EC') and L. esculentum × L. pimpinellifolium cv. `New York 280' (`NY'), differing in chilling tolerance, were compared before and after 16 days of chilling at 4°C, and after return of the fruit to 20°C for one and five days. Ripening parameters, color and texture, were monitored during the experiments. Color development showed normal ripening of chill-tolerant `NY' after the fruit were returned to 20°C, but failure of chill-sensitive `EC' to ripen. Before chilling, slight differences in the patterns of translation products resolved by 2D-PAGE were observed between the two cvs. probably reflecting their different genetic background. Chilling induced changes in gene expression that were for a large part similar in fruit of both cvs. However, among responses that differed in the two cvs., changes in the levels of two low-molecular-weight basic translation products identified as `A' (ca. 18 kDa, pH 8.0) and `B' (ca. 14 kDa, pH 8.2) were most noticeable. Translation product `A', faintly visible in `NY' before chilling, was strongly expressed after 16 days of chilling and 24 h after transfer to 20°C, but had disappeared five days after transfer to 20°C. Translation product `A' also increased in abundance in `EC' at low temperature and was retained at appreciable levels five days after the fruit were returned to 20°C. Translation product `B' highly expressed in chilling-tolerant `NY' before chilling, was sharply reduced in chilled fruit. This change was readily reversible upon transfer to 20°C. By contrast, the level of polypeptide `B' remained low in `EC' under all treatments.