Cell wall synthesis in kiwifruit following postharvest ethylene treatment.
Redgwell, R.J.
Phytochemistry. Vol: 41 Issue: 2 Pages: 407-413.
1996
บทคัดย่อ
Kiwifruits (Actinidia deliciosa) labelled by exposure to 14CO2 were examined for their capacity to synthesize cell wall polysaccharides immediately after harvest and during ripening. The biosynthetic ability of intact kiwifruit was compared with that of the corresponding fruit discs. Discs were treated in two ways. They were either allowed to soften over 72 hr after excision from 14CO2-labelled intact fruit or they were excised from unlabelled fruit and treated with 14C-glucose as an exogenous precursor. Intact fruit showed incorporation of radioactivity into cell wall material during ripening, but over 90% of radioactivity was in cell wall-associated protein. Radioactivity was detected in galactose, mannose and glucose from the cell wall material, but there was no ripening-related enhancement of the labelled components. Similar results were obtained with 14CO2-labelled discs. In contrast, discs labelled with 14C-glucose were able to incorporate a greater proportion of the radioactivity into cell wall polysaccharide with extensive labelling of all monosaccharide components of the cell wall. The amount of incorporated label increased over 72 hr, but there was no evidence for incorporation into different polysaccharides between 24 and 72 hr as discs softened. It is concluded that although mature and ripe kiwifruit are able to synthesize cell wall polymers during ripening it does not appear to be related to the softening process.