บทคัดย่องานวิจัย

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Chemical modification of essential arginine residues associated with the red beet (Beta vulgaris L.) plasma membrane Ca2+-ATPase.

Basu, S. and Briskin, D.P.

Phytochemistry. Volume 41, Number 3, Feb 1996. Pages 685-689.

1996

บทคัดย่อ

Chemical modification of essential arginine residues associated with the red beet (Beta vulgaris L.) plasma membrane Ca2+-ATPase.

Two alpha-dicarbonyl reagents, phenylglyoxal and 2,3-butanedione, were used to demonstrate the presence of essential arginine residues in the mechanism of the plasma membrane Ca2+-ATPase of red beet (Beta vulgaris L.) storage tissue. Both the ATP-dependent 45Ca2+ transport and ITP hydrolytic activities of the Ca2+-ATPase were inhibited by these reagents. Optimal inhibition was observed at pH 7.5 and 25 degrees. Inhibition of ATP dependent 45Ca2+ transport by phenylglyoxal and 2,3-butanedione was decreased by inclusion of ATP in the incubation medium. These results demonstrate that arginine residues are involved in the mechanism of the red beet plasma membrane Ca2+-ATPase and may reside at the ATP binding region of the enzyme active site.