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Monophenolase activity of strawberry polyphenol oxidase.

Espin, J.C., Ochoa, M., Tudela, J. and Garcia Canovas, F.

Phytochemistry -Oxford. Volume 45, Number 4, June 1997. Pages 667-670.

1997

บทคัดย่อ

Monophenolase activity of strawberry polyphenol oxidase.

Strawberry polyphenol oxidase (PPO) was extracted and partially purified by using three sequential phase partitionings with Triton X-114 (TX-114) reported here for the first time. Monophenolase activity of strawberry PPO was reported for the first time. The enzyme fulfilled all the tests of the kinetic reaction mechanism previously proposed for PPOs from other sources. Therefore, this mechanism was also applicable to monophenolase activity of strawberry PPO.