บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Effect of exogenous calmodulin and ATP on the activity of ethylene forming enzyme obtained from tomatoes and green pea pods.

Charles K Njoroge, Eduardo L Kerbel and Donald P Briskin

Journal of the Science of Food and Agriculture. Volume 76, Issue 2, Feb 1998. Pages 215-220.

1998

บทคัดย่อ

Effect of exogenous calmodulin and ATP on the activity of ethylene forming enzyme obtained from tomatoes and green pea pods.

Ethylene forming enzymes (EFE) from both peas and tomatoes were prepared. EFE from both sources were inhibited by adenosine triphosphate (ATP). Exogeneous calmodulin had no effect on EFE activity either in the presence or absence of ATP. The presence of gramicidin in pea microsomal membrance EFE did not affect EFE activity in the presence of ATP. This suggests that ATP is inhibiting EFE activity through phosphorylation and not through transmembrane proton flux. However, inhibition of EFE activity by ATP was partially reversed by 60 micromolar N-6(aminohexyl)-5-chloro-1-naphthalenesulphonamide (W7) and 15 micromolar trifluoperazine (TFP) but not by 60 micromolar N-6(aminohexyl)-1-naphthalenesulphonamide (W5), which suggests that the ATP inhibitory affect on EFE is probably controlled by calmodulin.