บทคัดย่องานวิจัย

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Some properties of gamma-tocopherol methyltransferase solubilized from spinach chloroplasts.

Ishiko, H., Shigeoka S, Nakano Y and Mitsunaga T.

Phytochemistry, Volume 31, Number 5, May 1992. pp. 1499-1500.

1992

บทคัดย่อ

Some properties of gamma-tocopherol methyltransferase solubilized from spinach chloroplasts.

gamma-Tocopherol methyltransferase occurs in the chloroplast fraction of spinach leaves. Its specific activity with gamma-tocopherol and S-adenosyl-L-methionine was 3.91 nmol hr-1 mg-1 protein. The enzyme was effectively solubilized by 6 mM sodium deoxycholate from the membrane fraction of chloroplasts. The activity was maximum at pH 7.5 and 35 degrees. gamma-Tocopherol was preferred to beta-tocopherol (25:7). The Km value for S-adenosyl-L-methionine as methyl donor was 9.1 micromolar.