Postharvest biological control of gray mold of apple by Cryptococcus laurentii.
Roberts, R. G.;
Phytopathology Year: 1990 Vol: 80 Issue: 6 Pages: 526-530 Ref: 21 ref.
1990
บทคัดย่อ
Puncture wounds in surface-disinfested Golden Delicious apple fruit were treated with phosphate buffer, cell suspensions of C. laurentii, or benomyl, then inoculated with 2 x 104 conidia/ml of Botrytis cinerea and incubated for 12 d at 5, 10, 15 or 20 deg C. Treatment of wounds with washed cells of C. laurentii at 104-105 cells/wound effectively reduced or prevented development of decay by B. cinerea at all temp. compared with controls and was comparable in effectiveness to preinoculation application of benomyl at the postharvest label rate. Treatment of wounds with cell-free culture filtrates of C. laurentii were not effective in preventing decay and resulted in greater lesion diam. than in inoculated, buffer-treated wounds. As the interval between wounding and inoculation with B. cinerea increased from 0 to 72 h, susceptibility of wounds to decay by B. cinerea decreased. Population densities of C. laurentii in wounds increased rapidly, even at 5 deg , and were never associated with necrosis or discoloration of host tissue.