ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

In vitro preservation at low temperature of rose plantlets usable for direct acclimatization.

Dorion, N.; Kadri, M.; Bigot, C.;

Acta Horticulturae Year: 1991 Issue: No. 298 Pages: 335-343 Ref: 6 ref.

1991

บทคัดย่อ

Shoot explants from rose plantlets (cultivars Mme Delbard and Eterna) were placed on MS rooting medium and stored immediately (R0) or after a short period in a growth chamber to promote root induction (6 days in the growth chamber, R6) or growth (12 days, R12). The rooting medium contained sucrose at 15, 30 or 45 g/litre. The explants were stored for 2, 4 or 6 months at 2 or 4 deg C in darkness or under dim fluorescent light (8 h at 2-3 W/m2). The explants were then acclimatized at 18-20 deg for 1 week and then at 20-25 deg for 2 weeks, with 90% RH and natural daylight supplemented with high intensity discharge lamp lighting. Plants from the R0 treatment were kept for 8 days in a growth chamber (for rooting) before acclimatization. Light during the explant storage period increased the number of roots initiated/explant compared with darkness, particularly for the 45 g sucrose/litre treatment. Plants stored for 2 or 4 months at 4 deg acclimatized better than plants stored at 2 deg . However, when stored for 6 months, acclimatization ability was highest after storage at 2 deg . The 45 g sucrose/litre treatment resulted in a significantly higher number of roots induced/explant, higher survival and increased plant growth in the acclimatization phase compared with 15 and 30 g sucrose/litre. For all storage periods, plantlet growth was highest in R0-treatment explants. Placing R0 explants in a growth chamber for 8 days before acclimatization improved the rooting percentage.