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The infiltration ability of Bacillus subtilis cells into xylem vessels of cut rose cv. Sonia: cryo-SEM observations.

Put, H. M. C.; Klop, W.; Clerkx, A. C. M.;

Acta Horticulturae Year: 1991 Issue: No. 298 Pages: 303-312 Ref: 6 ref.

1991

บทคัดย่อ

The infiltration ability of Bacillus subtilis cells into xylem vessels of cut rose cv. Sonia: cryo-SEM observations.

Cut roses (cv. Sonia) were kept in sterilized deionized water alone or with the addition of 500 micro g Al2(SO4)3/ml, B. subtilis cells (107 cells/ml) or Al2(SO4)3 + B. subtilis cells for 48 h. Cryo-SEM observations were made of transverse and longitudinal cross-sections of xylem vessels and X-ray microanalysis of stem pieces was carried out. Cryo-SEM observations showed that the cut surface of flower stems acted as a coarse threaded filter and only a small fraction of B. subtilis cells entered with the sapstream into the xylem vessels. The remaining fraction adhered to the cut surface or the lower part of the vessels. When Al2(SO4)3 was added to the vase water in addition to the B. subtilis cells, the cells were surrounded by an insoluble Al-compound which reduced the ability of the cells to infiltrate the xylem vessels. Al2(SO4)3 in the vase water formed flocks in the fluid and on the cut stem surface, but X-ray microanalysis showed that the Al-compounds were not taken up in the sapstream. The biocidal activity of the Al-compounds was negligible.