บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

The use of cryo-electron microscopy and cryo-ultramilling to investigate the occlusion in the xylem of cut flowers.

Doorn, W. G. van, Clerkx, A. and Boekestein, A.

Acta Horticulturae Year: 1991 Issue: No. 298 Pages: 183-188 Ref: 4 ref.

1991

บทคัดย่อ

The use of cryo-electron microscopy and cryo-ultramilling to investigate the occlusion in the xylem of cut flowers.

Segments from stems of cut flowering roses held in water for 2 days were cryofixed in nitrogen slush (at about 60 K), and ultramilled with a microtome equipped with a cryostage (at about 100 K), etched for 8 min at 20 Pa and 190 K, gold-sputtered at 100 K and examined at 120 K in a scanning electron microscope equipped with a cold stage. Compared with conventional techniques, the cryofixation method showed more of the amorphous substance accompanying individual bacteria and groups of bacteria in the xylem vessels, indicating the possible significance of bacterial extracellular polysaccharides in vascular occlusion. It has been suggested that bacteria might degrade the xylem cell walls resulting in blockage. However, cross sections of the walls, prepared by cryo-ultramilling, had a smooth, unaffected appearance.