In vitro regeneration of plants from stored Witloof chicory.
Mohamed-Yasseen, Y.; Splittstoesser, W. E.;
Plant Growth Regulator Society of America Quarterly Year: 1991 Vol: 19 Issue: 1 Pages: 41-45 Ref: 10 ref.
1991
บทคัดย่อ
A micropropagation procedure was developed to regenerate plants via tissue culture from explants of Cichorium intybus heads. All explants except lamina explants from the upper part of the leaf showed a high degree of bacterial contamination [unspecified]. About 50% of the shoots produced from lamina explants flowered in vitro under a 16-h photoperiod and produced viable seeds in the greenhouse. The procedure permits recovery of germplasm that may exhibit resistance to postharvest physiological disorders and enables the study of flowering in vitro. MS media supplemented with thidiazuron (0.05 or 0.1 micro M) or a combination of BA (8.0 micro M) and NAA (0.1 micro M) resulted in the successful regeneration of plants from explants.