ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Changes in glycosidase activities during development and ripening of melon.

Fils-Lycaon, B.; Buret, M.;

Postharvest Biology and Technology Year: 1991 Vol: 1 Issue: 2 Pages: 143-151 Ref: 27 ref.

1991

บทคัดย่อ

Changes in the activity of several glycosidases were studied in mesocarp tissues of muskmelon (cv. Alpha) during fruit ontogeny. Mesocarp tissue contained alpha - and beta -D-galactosidase, alpha -D-mannosidase, beta -D-glucosidase, beta -D-xylosidase, alpha -L-arabinopyranosidase and alpha -L-arabinofuranosidase activities at selected stages during fruit development. The activities of alpha -D-galactosidase and beta -D-xylosidase did not change significantly during ripening. The activities of alpha -D-mannosidase and alpha -L-arabinofuranosidase increased slightly from the turning to the ripe stages, while those of beta -D-galactosidase, alpha -L-arabinopyranosidase and beta -D-glucosidase increased significantly from the turning to the overripe stages. When expressed on a protein basis, all activities measured increased at the turning stage, but only those of beta -D-galactosidase, alpha -L-arabinopyranosidase and beta -D-glucosidase continued until the end of ripening and overripeness. Glycosidases with specific activities which increased or remained constant during the final stages of ripeness were mainly solubilized in a high-ionic-strength-extractable fraction. In contrast, those with specific activities which decreased towards full ripening were mainly solubilized using a low-ionic-strength extractant. A possible role for glycosidases in muskmelon softening is discussed.