Wound-induced ethylene synthesis and its involvement in enzyme induction in mesocarp tissue of Cucurbita maxima.
Hyodo, H.; Tanaka, K.; Suzuki, T.;
Postharvest Biology and Technology Year: 1991 Vol: 1 Issue: 2 Pages: 127-136 Ref: 17 ref.
1991
บทคัดย่อ
Peroxidase activity in mesocarp tissue of C. maxima fruits increased rapidly after wounding. The increase in peroxidase activity was preceded by increased ethylene production. The induction of peroxidase activity was strongly suppressed by the application of 2,5-norbornadiene and aminoethoxyvinylglycine to excised discs, indicating that endogenous ethylene produced in response to wounding may have promoted the induction of peroxidase. Raised activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and phenylalanine ammonia-lyase (PAL) were noted prior to increased peroxidase activity. Although no significant increases in soluble phenolics, such as chlorogenic acid, were found in the wounded mesocarp tissue, lignin formation was clearly enhanced by wounding. ACC synthase and PAL induced by wounding were located predominantly in the upper and adjacent 0.5 mm of the cut surface, while peroxidase activity was found to exist deep inside the tissue.