บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Reduction of adenosine triphosphate in eggs of Fuller rose beetle (Coleoptera: Curculionidae) induced by lethal temperature and methyl bromide.

Forney, C. F.; Aung, L. H.; Brandl, D. G.; Soderstrom, E. L.; Moss, J. I.;

Journal of Economic Entomology Year: 1991 Vol: 84 Issue: 1 Pages: 198-201 Ref: 15 ref.

1991

บทคัดย่อ

Reduction of adenosine triphosphate in eggs of Fuller rose beetle (Coleoptera: Curculionidae) induced by lethal temperature and methyl bromide.

The concn of adenosine triphosphate (ATP) in relation to the viability of eggs of Asynonychus godmani [Pantomorus cervinus] was determined with Photinus pyralis bioluminescent assay. ATP concn decreased significantly when eggs were killed by freezing, hot water or methyl bromide fumigation. Two hours after eggs were frozen in liquid nitrogen, the ATP concn was only 5% that of unfrozen, live eggs. The ATP concn decreased logarithmically for the first 30 min after freezing; the ATP half-life was estimated to be 12.9 min. ATP concn in eggs killed by a 3-min dip in 55 deg C water dropped 40% 2 h after treatment. In eggs fumigated for 2 h with 80 g/m3 methyl bromide, the ATP concn did not decrease for 8 h following treatment. However, after 24 h, ATP concn were 50% less than the nonfumigated eggs. The P. cervinus eggs killed by all 3 treatments contained significantly less ATP than live eggs 24 h following treatment. Thus, the results suggest that P. pyralis ATP assay could serve as a rapid screening method for ascertaining egg viability of P. cervinus.