The dynamics of bud development in ratooning stubble of sugar cane varieties.
Ferraris, R. and Chapman, L. S.
Proceedings of the Australian Society of Sugar Cane Technologists. Year: 1991 Issue: No. 13 Pages: 164-171 Ref: 4 ref.
1991
บทคัดย่อ
Cultural conditions were found to be important in determining the extent of Cx-cellulase activity in P. digitatum and P. italicum, post harvest decay pathogens of citrus. At pH 5.5-7.5, activity was correlated with mycelial dry wt. However, at pH 4.5 and more so at pH 3.5, activity was markedly reduced while fungal growth was not affected. Induction of Cx-cellulases of both species was delayed by the presence of carboxymethyl cellulose as a carbon source, indicating that the cellulases are constituent enzymes. Cellulase activity of the 2 species on different carbon sources was detected prior to the initiation of sporulation. A sporeless mutant of P. digitatum exhibited cellulolytic activity similar to that of the normal strain, suggesting no role for sporulation in the Cx-cellulase synthesis. Cx-cellulase activity in orange cv. Valencia started during the early stages of pathogenesis, before the appearance of symptoms. A correlation between cellulase activity and symptom severity was apparent during the first 2 d after inoculation. At the end of the incubation period both fungi almost attained their max. enzymic activity, whereas the disease index continued to rise gradually until total rot was achieved. A possible role of Cx-cellulases of the 2 species in the early stages of pathogenesis was suggested.