Micro-organisms at the cut surface and in xylem vessels of rose stems: a scanning electron microscope study.
Doorn, W. G. van; Stigter, H. C. M. de; Witte, Y. de; Boekestein, A.;
Journal of Applied Bacteriology Year: 1991 Vol: 70 Issue: 1 Pages: 34-39 Ref: 20 ref.
1991
บทคัดย่อ
Stems of cut rose flowers (Rosa hybrida L. cv. 'Sonia') were placed in water to study the development of a population of microorganisms at the cut surface and in the xylem vessels. The cut surface became covered with bacteria within 2 d of vase life. The bacteria were accompanied by an amorphous substance which was apparently bacterial slime. After 7 d of vase life many fungal hyphae were also found at the cut surface. Inside the xylem vessels the bacteria were often clustered at the inter-vessel pits. After 4 d of vase life most of the vessels that had been opened by cutting contained bacteria. Only a few xylem elements, located several centimeters from the cut surface, contained an amorphous substance. A few fungal hyphae were observed inside the xylem vessels. No yeasts were found, either at the cut surface or inside the xylem. Pseudomonas spp. accounted for > 70% of the total bacteria population of the cut surface and the xylem vessels, and Enterobacter spp. (mainly E. agglomerans) for < 10%. Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Citrobacter and Flavobacterium were occasionally observed.