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Distribution and amounts of taxol in different shoot parts of Taxus cuspidata.

Neto, A. G. F.; DiCosmo, F.;

Planta Medica Year: 1992 Vol: 58 Issue: 5 Pages: 464-466 Ref: 6 ref.

1992

บทคัดย่อ

Distribution and amounts of taxol in different shoot parts of Taxus cuspidata.

The dichloromethane extracts of T. cuspidata plants (collected from the Toronto University campus) were analysed for their taxol concentrations, using chromatographic and spectral analyses. Extracted parts included: young needles (first 10 top needle pairs of 30 cm long branches), old needles (last 10 needle pairs of 30 cm long branches), green bark, dark bark (with intense secondary growth), young wood (originally surrounded by green bark), wood (originally surrounded by dark bark), young stems (surrounded by green bark and devoid of needles), and mature male cones. All parts except for the male cones contained detectable amounts of taxol. Plant sex did not appear to affect taxol levels. The bark accounted for almost all the taxol present in stems devoid of needles. Needles showed the highest taxol concentration of 0.035 plus or minus 0.006% (of the extracted DW), while dark bark contained 0.012 plus or minus 0.001% on average. In studies on the effect of needle post-harvest procedures on taxolcontents, 96 h dark incubation at -12 or +25 deg C under vacuum resulted in taxol yields equivalent to those of freshly extracted samples; dark incubation at 60 deg enhanced taxol degradation.