บทคัดย่องานวิจัย

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Activities of enzymes involved in lignification during the postharvest storage of etiolated asparagus spears.

Hennion, S.; Little, C. H. A.; Hartmann, C.;

Physiologia Plantarum Year: 1992 Vol: 86 Issue: 3 Pages: 474-478 Ref: 32 ref.

1992

บทคัดย่อ

Activities of enzymes involved in lignification during the postharvest storage of etiolated asparagus spears.

The lignin content and activities of 5 enzymes involved in lignification were monitored along the length of etiolated spears of asparagus (INRA F1 male hybrid no. 156) stored for 22 h with their bases in air (control), water or water containing the ethylene antagonist, silver thiosulphate (STS). At the time of harvest, the lignin content was higher at the base of spears than at the apex, as was the activity of the enzymes studied, viz., phenylalanine ammonia lyase (PAL), hydroxycinnamate:CoA ligase (HCoAL), cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (CAD) and syringaldazine oxidase (SyrOx, a peroxidase with syringaldazine as substrate). Neither the lignin content nor the activity of any enzyme changed in the spear apex during storage, regardless of treatment. In the spear base, all enzyme activities decreased during the first 2 to 4 h in each storage treatment. Subsequently, PAL and HCoAL activities remained constant, whereas the activities of CAD and SyrOx gradually increased. Lignification in the spear base was not affected by storage in air. However, storage in water resulted in an increase in lignin formation and SyrOx activity, whereas treatment with STS prevented both of these increases. The results indicate that ethylene is involved in the regulation of postharvest lignification in etiolated asparagus spears by modulating SyrOx activity.