Biological control of postharvest diseases of grape, peach, and apple with the yeasts Kloeckera apiculata and Candida guilliermondii.
McLaughlin, R. J.; Wilson, C. L.; Droby, S.; Ben-Arie, R.; Chalutz, E.;
Plant Disease Year: 1992 Vol: 76 Issue: 5 Pages: 470-473 Ref: 30 ref.
1992
บทคัดย่อ
K. apiculata str. 138 was isolated from the surface of grapes and evaluated for its activity in reducing postharvest decay of grapes, peaches and apples. In an artificial infection assay in which detached grapes were immersed in an aqueous suspension of yeast cells (5 x 108 c.f.u./ml) and spray-inoculated with 103 sporangiospores/ml of Rhizopus stolonifer, str. 138 reduced decay, whereas str. 87 (= US-7) of C. guilliermondii did not. In an assay in which naturally infected fruits were immersed in yeast suspensions, both strains were effective in reducing postharvest decay of grapes caused by R. stolonifer; however, neither yeast was effective in reducing decay caused by Aspergillus niger. Three strains of K. apiculata obtained from other sources were compared with str. 138 and str. 87 for activity against Rhizopus rot of peach (caused by R. stolonifer) and grey mould of apple (caused by Botrytis cinerea). Fruit wounds were pretreated with water-suspended yeast cells (108 c.f.u./ml) and inoculated with 103 sporangiospores of R. stolonifer/ml or 105 conidia of B. cinerea/ml. Of 3 strains of K. apiculata tested, 2 were as effective as strains 138 and 87 for the control of Rhizopus rot of peach. All strains were equally effective in reducing grey mould of apple. Control of grey mould and blue mould (caused by Penicillium expansum) of apples and Rhizopus rot of peaches was enhanced when str. 138 was applied as an aqueous suspension (108 c.f.u./ml) in 2% CaCl2. This method did not reduce the incidence of brown rot of peach, caused by Monilinia fructicola; however, decreased lesion diam. was observed in fruit treated with yeast and CaCl2.