บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Use of antisense RNA technology to study pectin degradation in tomato fruit.

Tucker, G. A.; Seymour, G. B.; Bundick, Y.; Robertson, D.; Hall, L.; Smith, C. J. S.; Grierson, D.; Bird, C. R.; Schuch, W.;

New Zealand Journal of Crop and Horticultural Science Year: 1992 Vol: 20 Issue: 2 Pages: 119-124 Ref: 27 ref.

1992

บทคัดย่อ

Use of antisense RNA technology to study pectin degradation in tomato fruit.

Tomato plants (cv. Ailsa Craig) which were either normal (control) or homozygous antisense transformants for polygalacturonase (PG) or pectinesterase (PE) were grown in a glasshouse and fruits were harvested at different stages of ripening. Both enzymes are thought to be involved in the modification of polyuronides during ripening. PG is absent from green fruits but is synthesized during ripening; it occurs in at least 3 isoenzyme forms. Reduced PG was found to prevent pectin depolymerization during ripening and resulted in fruits with improved storage and transport characteristics. Reduced PE resulted in increased pectin esterification throughout ripening, but had no apparent effect on fruit physiology. All other aspects of pectin metabolism tested were unaffected in these transgenic fruits.