Molecular biological investigations of gene expression attending fruit ripening: current status and future prospects.
Dilley, D. R.; Wilson, I. D.;
HortTechnology Year: 1992 Vol: 2 Issue: 3 Pages: 294-301 Ref: 47 ref.
1992
บทคัดย่อ
The subject is reviewed, with emphasis on ripening in tomatoes, under the following headings: concepts and protocols of molecular biology; biochemistry and enzymology of fruit ripening; gene expression of ACC oxidase; and future prospects for molecular biological studies of gene expression of ripening. Studies on gene expression have revealed that development of the ethylene biosynthetic pathway is transcriptionally regulated through expression of respective mRNAs encoding ACC synthase and ACC oxidase, and that this process is regulated by ethylene. This work has led to the development of genetically engineered tomato plants in which the enzymes of ethylene biosynthesis and the fruit softening enzyme polygalacturonase have been attenuated, resulting in delayed fruit ripening. However, many of the developmental aspects of ripening are still poorly understood.