บทคัดย่องานวิจัย

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Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea.

Bossi, R.; Dewey, F. M.;

Plant Pathology Year: 1992 Vol: 41 Issue: 4 Pages: 472-482 Ref: 24 ref.

1992

บทคัดย่อ

Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea.

Three hybridoma cell lines secreting antibodies that specifically recognize B. cinerea and B. fabae, but not B. allii, were raised from splenocytes of mice immunized with a low molecular-weight fraction (30 kDa) from surface washings of B. cinerea. Antibodies from these cell lines were used to develop an antigen-based ELISA to detect B. cinerea in strawberries. This monoclonal antibody immunoassay detection assay should prove useful to both the cut-flower and wine industries. Supernatants from the 3 specific cell lines recognize mycelial fragments, saline extracts of mycelia and germinating conidia by both ELISA and immunofluorescence. Recognition of non-germinating spores is poor. Supernatants from the specific cell lines did not recognize other fungi normally involved in postharvest spoilage of fruits and vegetables. Supernatants from KH4 gave the lowest background values with healthy tissue. Indirect evidence from heat, protease and periodate treatment of the antigens indicates that antibodies from all 3 specific cell lines are recognizing carbohydrate epitopes on a glycoprotein.