Monoclonal antibodies for the detection of spoilage fungi.
Dewey, F. M.; Banham, A. H.; Priestley, R. A.; Martin, B.; Hawes, C.; Phillips, S. I.; Wareing, P. W.;
International Biodeterioration & Biodegradation Year: 1993 Vol: 32 Issue: 1-3 Pages: 127-136 Ref: 24 ref.
1993
บทคัดย่อ
The use of monoclonal antibodies (MAbs) in the development of immunoassays to detect the mycelium of specific fungi in foods and feed are reviewed. The development of MAb-ELISA and "user-friendly" dip stick assays for the detection of Humicola lanuginosa and Penicillium islandicum, involved in postharvest discoloration of rice grains is described, as is a MAb-ELISA for the detection of Botrytis cinerea in extracts from infected strawberries and grapes. These assays are reported to be sensitive, rapid, easily replicated, do not require surface sterilization and detect only mycelial growth. Difficulties in raising species-specific MAbs to fungi, particularly those related to choice of immunogen, are discussed.