บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Postharvest inhibition of glutamine synthetase activity with phosphinothricin reduces the shelf-life of asparagus.

Hurst, P. L.; King, G. A.; Borst, W. M.;

Postharvest Biology and Technology Year: 1993 Vol: 3 Issue: 4 Pages: 327-334 Ref: 23 ref.

1993

บทคัดย่อ

Postharvest inhibition of glutamine synthetase activity with phosphinothricin reduces the shelf-life of asparagus.

Harvested spears of asparagus accumulate ammonia (or ammonium ion) in their tips towards the end of their shelf-life. Experiments with harvested spears (cv. Limbras 10) indicated that the accumulation of ammonia is not due to limitations in the activity of the ammonia assimilating enzyme, glutamine synthetase (GS). When GS was inhibited by postharvest treatment with phosphinothricin (PPT), the ammonia content of the tips increased almost immediately and the shelf-life of the spears was severely reduced with symptoms of deterioration identical to those commonly ascribed to chilling injury. PPT treatment also inhibited the pattern of glutamine and asparagine accumulation observed during shelf-life. These observations emphasize the pivotal role of GS in reassimilating considerable amounts (3% of DW) of ammonia in the tips of harvested spears.