Action of Cx-cellulase on avocado cell wall polymers.
O'Donoghue, E. M.; Huber, D. J.; Timpa, J. D.;
Acta Horticulturae Year: 1993 Issue: No. 343 Pages: 212-214 Ref: 7 ref.
1993
บทคัดย่อ
The physiological role of Cx-cellulase in the loss of fruit firmness was investigated in pre-ripe and edibly soft avocado fruits (cv. Lula). Gel permeation chromatography of total cell wall polysaccharides (including cellulose) showed a proportional increase in the MW of the largest polymers during fruit ripening. When enzymically inactive cell walls from unripe fruits were exposed to Cx-cellulase, there was a gradual erosion of the largest polymers and a subsequent lowering of the average MW of this peak. It is suggested that avocado Cx-cellulase preferentially attacks the amorphous regions of microfibrils resulting in a gradual decrease in cellulose MW and a change in the microfibril interaction with surrounding matrix material. Such activity could influence the integrity of the cell wall structure and have a direct effect on the loss of fruit firmness.