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Production of methanethiol by anaerobic broccoli and microorganisms.

Forney, C. F.; Hildebrand, P. D.; Saltveit, M. E.;

Acta Horticulturae Year: 1993 Issue: No. 343 Pages: 100-104 Ref: 17 ref.

1993

บทคัดย่อ

Production of methanethiol by anaerobic broccoli and microorganisms.

Fresh broccoli held in low O2 atmospheres develops an objectionable off-odour. The primary chemical responsible for this off-odour has been identified as methanethiol (MT). This study was carried out to investigate whether MT from anaerobic broccoli originates from plant tissues or microorganisms or both. Bacteria were isolated from healthy broccoli florets and isolates were cultured on Pseudomonas Agar F in test tube slants. Cultures were sealed, flushed with N2 and after 24 h the headspace in the tubes was analysed for MT using GC. Sterile broccoli seedlings (cultivars Premium Crop, Shogun, Arcadia and Waltham 29) were grown in jars. After 4-8 weeks, jars with sterile seedlings were sealed and flushed with N2. After 24 h at 20 deg C the headspace was analysed for MT. Of the 56 isolates of aerobic bacteria from broccoli florets, 62% produced MT, 16% produced methyl sulphide and 9% produced both MT and methyl sulphide. Sterile broccoli seedlings produced MT when they were sealed and flushed with N2. H2S was also detected in the sealed jars and its concentration increased over time. Plants sealed in air produced no MT (unlike bacteria) or H2S.