Cellular compartmentation and transport into tonoplast vesicles of sugars with ripening of pear fruit.
Yamaki, S.; Ino, M.; Ozaki, S.; Tsuchimoto, Y.; Ofosu-Anim, J.;
Acta Horticulturae Year: 1993 Issue: No. 343 Pages: 12-17 Ref: 11 ref.
1993
บทคัดย่อ
The distribution of sugars into vacuoles, cytoplasm and free spaces during maturation and ripening of pear fruits (cv. La France) was studied using a compartmental analysis method. More than 80% of the sugars were located in the vacuoles of the fruit at each stage. There were no differences among the composition of fructose, sorbitol, sucrose and glucose in each compartment. Sugars accumulating in each compartment increased with fruit maturation. During fruit ripening, more sugars were deposited in the cytoplasm and free spaces and distribution of sugars into the vacuoles decreased. This increase in sugars in the free spaces during ripening may be due to the enhancement of permeability of sugars across the tonoplast and plasma membrane. The tonoplast vesicles isolated from mature pear fruits can take up sugar through the carrier-mediated transport system in which the uptake of sorbitol and fructose is coupled with H+-ATPase. Sugars translocated into the cytoplasm may be taken up actively into vacuoles across the tonoplast by this system.