Inhibition of auxin-induced ethylene production in cucumber fruit discs by carbon dioxide.
Mathooko, F. M.; Kubo, Y.; Inaba, A.; Nakamura, R.;
Postharvest Biology and Technology Year: 1993 Vol: 3 Issue: 4 Pages: 313-325 Ref: 34 ref.
1993
บทคัดย่อ
The induction of ethylene biosynthesis by indole-3-acetic acid (IAA) and its regulation by CO2 were examined in cucumber (cv. Sharp-1) fruit discs. Treatment of discs with IAA significantly stimulated ethylene production; the extent depended on IAA concentration, up to 1 mM. The increase in IAA-induced ethylene production was accompanied by increases in the activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase (ethylene-forming enzyme) and a large accumulation of ACC and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). The induction of ethylene production by IAA was inhibited by treatment with CO2. The extent of inhibition was dependent upon the concentration of CO2. The results indicated that CO2 inhibited IAA-induced ethylene biosynthesis by suppressing ACC synthase and ACC oxidase activities. Additionally, CO2 inhibited ACC conjugation into MACC.