บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Cloning of ethylene biosynthetic genes involved in petal senescence of carnation and petunia, and their antisense expression in transgenic plants.

Michael, M. Z., Savin, K. W., Baudinette, S. C., Graham, M. W., Chandler, S. F., Lu, C. Y., Caesar, C., Gautrais, I., Young, R., Nugent, G. D., Stevenson, K. R., O'Connor, E. L. J., Cobbett, C. S. and Cornish, E. C.

Proceedings of the International Symposium on Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylene. J.C. Pech, A. Latche, C. Balague (eds). Agen, France, August 31 - September 4, 1992. Year: 1993. p. 289-303.

1993

บทคัดย่อ

Cloning of ethylene biosynthetic genes involved in petal senescence of carnation and petunia, and their antisense expression in transgenic plants.

Ethylene regulates the processes of petal senescence in both carnation and petunia [Petunia hybrida]. To investigate the relationship between transcriptional regulation of ethylene production and petal senescence, genes involved in ethylene biosynthesis from the petals of the species have been cloned. Using the polymerase chain reaction a cDNA clone encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase from petunia has been isolated. Heterologous screening of a senescing petal cDNA library provided an ACC synthase encoding clone from carnation. Increased amounts of the mRNAs hybridizing to these cDNA clones are observed in climacteric petals and can be correlated with the onset of senescence. A cDNA clone encoding ACC oxidase was isolated by differential screening of a senescing carnation petal library. Antisense expression of ACC oxidase in transgenic carnation plants inhibits petal inrolling, increasing the postharvest life of the flowers.