บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Changes in protein and mRNA expression during cold storage of 'Cox's Orange Pippin' apple fruit.

Cregoe, B. A.; Ross, G. S.; Watkins, C. B.;

Acta Horticulturae Year: 1993 Issue: No. 326 Pages: 315-323 Ref: 20 ref.

1993

บทคัดย่อ

Changes in protein and mRNA expression during cold storage of 'Cox's Orange Pippin' apple fruit.

Cox's Orange Pippin apple fruits were held at either 0 deg or 4 deg C for up to 12 weeks. Some fruits were assessed immediately after removal from storage or held at 20 deg for 14 days. At intervals during cold storage, fruit quality (internal ethylene, background colour and flesh firmness) was assessed and protein and RNA extracted from samples of cortical tissue. Changes in the protein populations were examined by SDS-PAGE, and RNA was probed with ripening-related and cold-inducible cDNA clones. Fruits held at 0 deg were firmer and greener and had lower internal ethylene concentrations than those held at 4 deg , but fruits at 0 deg developed low temperature breakdown. There were few changes in protein populations in response to low temperature. Specific changes in RNA expression were found, both between temperatures and during storage at either temperature. Expression of RNA complementary to pAAS4 and pAP4 (clones associated with ethylene biosynthetic enzymes) increased during storage and was greater at 4 deg than at 0 deg . Expression of RNA complementary to one of 3 cold-regulated clones from barley and a cold-induced clone from pear fruits was also detected. The results indicate that changes in gene expression occur in apple fruits under cold storage conditions. Such changes may contribute to cold tolerance.