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Polygalacturonase isozymes from Botrytis cinerea grown on apple pectin.

Tobias, R.; Conway, W.; Sams, C.;

Biochemistry and Molecular Biology International Year: 1993 Vol: 30 Issue: 5 Pages: 829-837 Ref: 23 ref.

1993

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Polygalacturonase isozymes from Botrytis cinerea grown on apple pectin.

Five isoenzymes (4 acidic and 1 basic) of polygalacturonase were separated by chromatofocusing from the culture filtrate of B. cinerea, the causal agent of grey mould in postharvest apples, grown on apple pectin. The isoenzymes, designated as polygalacturonase I to V, had isoelectric points of 9.7, 4.9, 4.6, 3.7, and 2.7, respectively, with polygalacturonase III exhibiting the greatest specific activity. Polygalacturonase I appeared to function as an endo-polygalacturonase while the other 4 isoenzymes acted as exo-polygalacturonases. The pH optima of the isoenzymes ranged from pH 4.5 to 5.5 with polygalacturonase V being less sensitive to increased pH compared with the other isoenzymes.