Low O2-induced changes in pH and energy charge in pear fruit tissue.
Nanos, G. D.; Kader, A. A.;
Postharvest Biology and Technology Year: 1993 Vol: 3 Issue: 3 Pages: 285-291 Ref: 21 ref.
1993
บทคัดย่อ
Mature-green pear, cv. Bartlett [Williams' Bon Chretien], fruits were stored at 0 deg C for 2 or 4 weeks. After storage the fruits were ripened in air at 20 deg or treated for 4 days with 0.25% O2 at 20 deg before transfer to air for ripening. The juice pH (indicator of vacuolar pH) of air-treated fruits decreased slightly with ripening. Exposure to 0.25% O2 resulted in higher juice pH during the treatment and after transfer to air. Fruit discs, after 10 h aging, were also kept in air or 0.25% O2 at 20 deg for 2 days and their 31P-NMR spectra obtained. In discs treated with 0.25% 02, inorganic P concentration was higher in cytoplasm than in vacuoles, cytosolic pH was about 0.4 pH units lower and the ATP:ADP ratio was lower indicating lower energy charge. The above changes could alter the in vivo activity of a number of respiratory enzymes in fruits subjected to hypoxic treatments.