บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Effects of plant extracts on the in vitro and in vivo development of fruit pathogens.

Sardsud, U.; Sardsud, V.; Sittigul, C.; Chaiwangsri, T.;

Development of postharvest handling technology for tropical tree fruits: a workshop held in Bangkok, Thailand, 16-18 July 1992. Year: 1994 Pages: 60-62 Ref: 3 ref.

1994

บทคัดย่อ

Effects of plant extracts on the in vitro and in vivo development of fruit pathogens.

ABSTRACT

The antifungal activity of Acorus calamus, garlic, Centella asiatica, Cyperus rotundus, Languas galanga and Rhinacanthus nasutus occurring in Thailand were assessed. Twenty grams of each plant were ground and suspended in 100 ml of ethanol for 5 d before filtering to remove plant tissue. Using a rotary evaporator, the extracts were reduced to 10 ml and retained as test concentrates. Potato dextrose agar (PDA) was amended with the test extracts at 0.001, 0.01, 0.1 and 1.0% prior to pouring the plates. The plates were then inoculated with Lasiodiplodia [Botryodiplodia] theobromae and incubated at 25-35 deg C and mycelial growth was measured daily. Only A. calamus extract at 1.0% completely inhibited the growth of B. theobromae. The antifungal activity of A. calamus was tested in vivo, by immersing longan fruit in 0.5 and 1.0% dilutions. The treated fruit were air-dried and plastic-wrapped in punnets, with fruit dipped in distilled water and undipped fruit serving as control treatments. The treated

 fruit were then stored at either 25, 10 or 5 deg and observed every second day. The extract showed no antifungal activity in vivo, with a similar spectrum of fungi to that reported previously associated with surface discoloured areas.