บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Effects of carbon dioxide on ethylene-forming enzyme in Japanese pear and apple.

Tian, M. S.; Hewett, E. W.; Lill, R. E.;

Postharvest Biology and Technology Year: 1994 Vol: 4 Issue: 1-2 Pages: 1-12 Ref: 23 ref.

1994

บทคัดย่อ

Effects of carbon dioxide on ethylene-forming enzyme in Japanese pear and apple.

ABSTRACT :

 

Ethylene-forming enzyme (EFE) activity was stimulated by CO2 in Japanese pear (cv. Hosui) and apple (cv. Granny Smith) fruit discs. In Hosui discs, EFE activity remained unchanged up to 5% CO2, but increased with higher CO2 levels up to 38% CO2. In Granny Smith discs, EFE increased gradually as CO2 levels increased from 5% to 30%. EFE activity was measured in discs from fruits removed from cold storage (1 deg C) at various intervals. EFE activity developed with storage time in both fruits. Treating discs with cycloheximide (protein synthesis inhibitor) showed that CO2 induced EFE biosynthesis in Granny Smith fruits stored for a short time, but not in Hosui fruits. Kinetic data from the interaction of CO2 and 1-aminocyclopropane-1-carboxylic acid (ACC) on EFE activity indicated that CO2 increased the maximum reaction rate (Vmax) of EFE in discs of both fruits without changing the apparent Michaelis-Menten constant (Km) value. It is suggested that the stimulatory effect of CO2 on EFE activity may

be caused by a CO2-EFE-ACC or EFE-ACC-CO2 complex which enhances the maximum reaction rate of conversion of ACC to ethylene.