A rapid immunoassay method for quantifying mold in harvested wine grapes.
Marois, J. J.; Kenyon, L. H.; Lamison, C. D.; Smith, J. P.;
American Journal of Enology and Viticulture Year: 1994 Vol: 45 Issue: 3 Pages: 300-304 Ref: 9 ref.
1994
บทคัดย่อ
ABSTRACT :
A rapid immunoassay developed by the University of California, Davis, and E. I. Dupont de Nemours was compared to the visual inspection assay presently used by the California Department of Agriculture to determine the amount of mold (Botrytis cinerea) in harvested wine grapes. Over 1000 trucks were sampled with both assays during the 1991 and 1992 harvests from inspection stations in the Central Coast, Livingston, Woodbridge, Ripon and North Coast wine grape production areas in California. The Pearson correlation coefficient (R) between the average of the visual assays and immunoassay was 0.99 and 0.97 for 1991 and 1992, respectively. Analysis of variance of the similarity of the 2 assays indicated that it was significantly affected by method of harvest (machine or hand), but not significantly affected by colour, site, variety, or time of harvest. In 1992, when enough data were obtained for analysis, the correlation between the 2 assays was significant at all sites except North Coast. This was p
robably due to the very low level of mold detected at the North Coast site with the visual assay (0.24%). At the other sites, R ranged from 0.66 to 0.76. The lowest correlation of the 2 assays was with machine-harvested black grapes (R = 0.23), while the R values for hand-harvested white and black grapes were 0.72 and 0.73, respectively, and 0.73 for machine harvested white grapes; this contrast was concluded to be due to the difficulty of visually detecting diseased berries in machine harvested black grapes. Frequency distribution analyses indicated that the visual assay agreed closely with the immunoassay, usually resulting in a variance:mean ratio of less than one.