Post-harvest rots of tomato in relation to lyases and mycotoxin production in vitro and in vivo.
Omar, S. A.; Mahmoud, A. L. E.;
Cryptogamie, Mycologie Year: 1994 Vol: 15 Issue: 4 Pages: 273-281 Ref: 25 ref.
1994
บทคัดย่อ
ABSTRACT :
A total of 220 isolates were recovered on Czapek's dextrose agar medium from 100 fruit samples showing rot symptoms, comprising 6 species, among which Alternaria alternata, Aspergillus flavus and A. niger were predominant, followed by Aspergillus tamarii, Cochliobolus spicifer and Penicillium citrinum. Testing enzymatic abilities of the isolates showed that the majority produced cellulase, pectin lyase and polygalacturonase on synthetic media and on inoculated tomatoes. Some isolates, which did not show enzymatic activities on agar media, produced enzymes on tomatoes. The majority of A. alternata, A. flavus and A. niger isolates tested were good enzyme producers and showed high enzymatic activity in vitro or in vivo. Healthy fruits had no detectable enzymatic activity. During screening for mycotoxigenicity, A. alternata produced the greatest number of mycotoxins in liquid medium and in infected tomatoes. In addition to A. alternata toxins, aflatoxins B1 and B2, and citrinin were produced by A. f
lavus and P. citrinum, respectively. The health hazard from these mycotoxins is discussed.