Survival of Verticillium albo-atrum in alfalfa seeds.
Huang, H. C.; Kozub, G. C.; Kokko, E. G.;
Canadian Journal of Botany Year: 1994 Vol: 72 Issue: 8 Pages: 1121-1125 Ref: 21 ref.
1994
บทคัดย่อ
ABSTRACT :
Hard seeds of alfalfa [lucerne] were artificially inoculated with V. albo-atrum and assessed for survival of the pathogen under lab. and field conditions. When infected seeds were stored air-dry, V. albo-atrum survival was low (< 10% after 10 months) at above-freezing temp. (20 and 30 deg C), but high (> 90% at 10 months) at subfreezing temp. (-20 and -10 deg ). Survival of the pathogen was also low (10% at 3 months) in seeds buried in soil at 20 deg but high (90% at 10 months) at -5 deg . More than 75% of the hard seeds remained viable after 18 months of air-dry storage at -20, -10, 4, 20 or 30 deg . The incidence of Verticillium wilt in lucerne plants due to seedborne V. albo-atrum decreased with seed storage period, averaging 40% after seed was stored for 1 month and 0% when seed was stored for 12 or 18 months. In field experiments, survival of seedborne V. albo-atrum decreased with increasing period of burial but at different rates in each year. The percentage of seeds with viable V. albo-at
rum was greater when the seeds were buried at 10 cm compared with 5 and 2 cm (48 vs. 39 and 38%, respectively). The complete loss of viability of V. albo-atrum in infected seeds stored at 30 deg for 6 months indicated that seed-borne V. albo-atrum could be eliminated by storing lucerne seeds at 30 deg for 1 year.