บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Cloning, antisense RNA inhibition, and the coordinated expression of UDP-glucose pyrophosphorylase with starch biosynthetic genes in potato tubers.

Spychalla, J. P.; Scheffler, B. E.; Sowokinos, J. R.; Bevan, M. W.;

Journal of Plant Physiology Year: 1994 Vol: 144 Issue: 4/5 Pages: 444-453 Ref: 42 ref.

1994

บทคัดย่อ

Cloning, antisense RNA inhibition, and the coordinated expression of UDP-glucose pyrophosphorylase with starch biosynthetic genes in potato tubers.

ABSTRACT :

 

A UDP-glucose pyrophosphorylase (UDPG-PPase, E.C.2.7.7.9) [UTPglucose-1-phosphate uridylyltransferase] cDNA from potato (Solanum tuberosum) tubers was cloned and sequenced. RNA analysis revealed expression in all tissues of the developing potato plant and that UDPG-PPase RNA could be induced by high sucrose treatments to excised leaves. In tubers, a coordinated expression of UDPG-PPase RNA with the RNA levels of several additional enzymes involved in the conversion of sucrose to starch was observed at harvest. Tubers of potato plants transformed with a patatin promoter-antisense-UDPG-PPase cDNA fusion were stored for 0, 1, 3 and 6 weeks at 4 deg C and 12 deg C. The tubers expressed various levels of antisense and sense UDPG-PPase RNA. The greatest antisense-mediated inhibition of UDPG-PPase enzyme activity was 30%, and this inhibition was maintained throughout the six weeks of storage at both 4 deg C and 12 deg C. No significant changes were consistently found in content of glucose, fructose, gl

ucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate and UDP-glucose in the transgenic tubers compared to wild type tubers. However, a statistically significant decrease in sucrose content was associated with the transgenic reduction of UDPG-PPase activity after 3 and 6 weeks of storage.